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Recovery & Repair Research Guide

TB-500: Dosage, Benefits & Research Guide

Also known as: Thymosin Beta-4 fragment, TB4-Frag

Key Facts

TB-500 is a recovery & repair research peptide (C212H350N56O78S, MW 4963.44 g/mol). Synthetic 43-amino acid peptide for tissue repair and anti-inflammatory research. It is supplied as a lyophilized powder for laboratory and in-vitro research use only — not for human consumption.

Classification Synthetic peptide fragment of thymosin beta-4
Molecular Formula C212H350N56O78S
Molecular Weight 4963.44 g/mol
CAS Number 77591-33-4
Research Half-Life Short circulating half-life in preclinical models
Form Lyophilized powder
Research Category Recovery & Repair

What is TB-500?

TB-500 is the synthetic version of Thymosin Beta-4, a naturally occurring 43-amino acid peptide present in virtually all human and animal cells. Its primary mechanism of action centers on sequestering G-actin monomers, which promotes actin polymerization and cell migration essential for tissue repair. TB-500 also upregulates expression of laminin-5, a key component of the basement membrane, and activates Akt (protein kinase B) signaling, which research indicates promotes cell survival and inhibits apoptosis. Published studies have demonstrated TB-500's wound healing properties in dermal repair models (Malinda et al., Journal of Investigative Dermatology, 1999) and corneal epithelium regeneration. In equine research, Thymosin Beta-4 has been extensively studied for tendon and ligament repair, with published veterinary trials showing accelerated recovery timelines. The peptide's anti-inflammatory properties appear to operate through suppression of NF-kB and reduction of pro-inflammatory cytokines including IL-1beta and TNF-alpha. Compared to BPC-157, TB-500 has a broader tissue distribution and systemic migration capability, making it a preferred research compound for widespread inflammatory conditions rather than localized injury models. The two peptides target complementary repair mechanisms, which is why they are frequently combined in research protocols. Store lyophilized powder at -20C; reconstitute with bacteriostatic water and refrigerate at 2-8C for up to 28 days. TB-500 is investigated by regenerative medicine centers, veterinary research institutions, and cardiology labs studying post-ischemic tissue recovery.

TB-500 Research Applications

In published and preclinical research, TB-500 has been studied across the following areas:

  • Angiogenesis and blood vessel formation
  • Wound closure and tissue regeneration
  • Collagen synthesis and scar reduction
  • Anti-inflammatory activity in joints

TB-500 in Research: Reconstitution & Study Concentrations

TB-500 corresponds to an active fragment of thymosin beta-4, a G-actin-sequestering peptide studied for cell migration, angiogenesis and tissue-repair endpoints. Preclinical protocols express concentrations per model system; there is no established human dose. The lyophilized peptide is reconstituted with bacteriostatic water for laboratory use. Research use only — not for human consumption.

Worked example: a 10mg vial reconstituted with 2 mL of bacteriostatic water yields 5.00 mg/mL.

Open the reconstitution calculator

How TB-500 Compares

Researchers frequently evaluate TB-500 alongside related compounds:

TB-500 — Frequently Asked Questions

What is TB-500 (Thymosin Beta-4) and how does it work?
TB-500 is a synthetic version of Thymosin Beta-4, a naturally occurring 43-amino acid peptide found in high concentrations in wound fluid, blood platelets, and other tissues. It functions by sequestering G-actin monomers to promote cell migration, proliferation, and differentiation. TB-500 has also been observed to upregulate actin polymerization, which is essential for angiogenesis and tissue remodeling in preclinical research models.
What research has been done on TB-500?
Published research in the Annals of the New York Academy of Sciences identified Thymosin Beta-4 as a key regulator of actin dynamics and wound healing. Studies in FASEB Journal demonstrated cardiac repair following ischemic injury in mouse models. Additional research has examined its role in hair follicle stem cell activation, corneal wound healing, and anti-fibrotic activity in liver and kidney injury models.
How does TB-500 compare to BPC-157?
TB-500 and BPC-157 target tissue repair through complementary mechanisms. TB-500 works primarily through actin regulation and cell migration, making it particularly studied for cardiovascular tissue, hair follicle, and dermal repair. BPC-157 acts through the NO system and growth factor modulation, with stronger gastrointestinal and tendon-specific research data. Their distinct pathways explain why researchers frequently study them together.
What is the recommended reconstitution protocol for TB-500?
For this 10mg vial, add 2 mL of bacteriostatic water to yield a concentration of 5.0mg/mL. Draw 10 units on a 1mL insulin syringe for a 500 mcg dose. Inject BAC water slowly along the inner vial wall — do not shake. Allow the powder to dissolve fully (2–5 minutes). Administer in the AM, every day. Refrigerate reconstituted solution at 2–8°C and use within 30 days.
Is TB-500 legal to buy for research?
TB-500 is sold in the United States as a research chemical for laboratory and in-vitro use only. It is not approved by the FDA for human use and is not sold for human consumption. Researchers are responsible for compliance with all applicable federal, state, and institutional regulations.
How is TB-500 reconstituted for research use?
TB-500 ships as a lyophilized (freeze-dried) powder and is reconstituted with bacteriostatic or sterile water before use in research. The volume of solvent added determines the working concentration — use the Elyte reconstitution calculator to derive the exact concentration for a given vial size and solvent volume.
Does TB-500 come with a Certificate of Analysis?
Yes. Every batch of TB-500 from Elyte Peptides ships with a third-party Certificate of Analysis (COA) documenting identity and HPLC purity (≥98%), so research results can be traced to a verified lot.

Research References

  1. Goldstein AL et al. Thymosin beta4: a multi-functional regenerative peptide. Expert Opin Biol Ther.
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