Weight Management Research Guide

5-Amino-1MQ: Dosage, Benefits & Research Guide

Key Facts

5-Amino-1MQ is a weight management research peptide (C10H11N2O, MW 175.21 g/mol). NNMT modulator for metabolic and weight management research. It is supplied as a lyophilized powder for laboratory and in-vitro research use only — not for human consumption.

Classification	NNMT modulator for metabolic and weight management research.
Molecular Formula	C10H11N2O
Molecular Weight	175.21 g/mol
Research Half-Life	Not established in published literature
Form	Lyophilized powder
Research Category	Weight Management

What is 5-Amino-1MQ?

5-Amino-1MQ (5-amino-1-methylquinolinium) is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that methylates nicotinamide and depletes the cellular pool of S-adenosyl methionine (SAM), a critical methyl donor. By inhibiting NNMT, 5-Amino-1MQ is proposed to increase intracellular NAD+ levels and SAM availability, thereby enhancing cellular energy metabolism and epigenetic regulation. Research published by Neelakantan et al. (Biochemical Pharmacology, 2018) demonstrated that NNMT inhibition in diet-induced obese mice reduced body weight and white adipose tissue mass while increasing energy expenditure, without changes in food intake. The same research group at the University of Texas showed that NNMT expression is upregulated in visceral adipose tissue of obese subjects, suggesting the enzyme plays a direct role in metabolic dysfunction. Unlike GLP-1 receptor agonists that suppress appetite centrally, 5-Amino-1MQ operates at the cellular metabolic level by modulating the NAD+ salvage pathway and methylation balance, offering a mechanistically distinct approach. Compared to NMN or NR supplementation (NAD+ precursors), 5-Amino-1MQ addresses NAD+ depletion at the enzymatic level rather than through precursor loading. Studies also suggest NNMT inhibition may improve insulin sensitivity through enhanced SIRT1 activity. Store lyophilized powder at -20C protected from moisture; reconstitute in sterile water as needed. This compound is investigated by metabolic biochemistry laboratories, NAD+ biology researchers, and academic institutions studying the intersection of epigenetics and obesity.

5-Amino-1MQ Research Applications

In published and preclinical research, 5-Amino-1MQ has been studied across the following areas:

Obesity and metabolic syndrome studies
Insulin resistance and type 2 diabetes research
NAD+ optimization for anti-aging
Energy and endurance performance

5-Amino-1MQ in Research: Reconstitution & Study Concentrations

5-Amino-1MQ is supplied as a lyophilized powder. For laboratory research it is reconstituted with bacteriostatic or sterile water; the solvent volume sets the working concentration. Published studies select concentrations specific to the assay or model system — there is no human dose, as 5-Amino-1MQ is not approved for human use. Researchers should reference the primary literature for the model in question and document the exact lot COA.

Worked example: a 5mg vial reconstituted with 2 mL of bacteriostatic water yields 2.50 mg/mL.

Open the reconstitution calculator

5-Amino-1MQ — Frequently Asked Questions

What is 5-Amino-1MQ and how does it work?

5-Amino-1MQ (5-amino-1-methylquinolinium) is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that regulates cellular energy metabolism and NAD+ levels. By inhibiting NNMT, 5-Amino-1MQ has been observed in preclinical models to increase intracellular NAD+ concentrations and activate the sirtuin (SIRT1) pathway, influencing fat cell metabolism, energy expenditure, and metabolic homeostasis.

What research has been done on 5-Amino-1MQ?

Research published in Biochemical Pharmacology demonstrated that 5-Amino-1MQ inhibited NNMT activity in adipocyte models, leading to reduced fat cell size and lipid accumulation. Studies at the University of Texas Health Science Center showed that NNMT inhibition by this compound class increased SAM (S-adenosylmethionine) concentrations and decreased fat mass in diet-induced obesity models without affecting food intake.

How does 5-Amino-1MQ compare to other NNMT inhibitors?

5-Amino-1MQ is among the most potent and selective NNMT inhibitors studied to date. Compared to earlier NNMT inhibitors like JBSNF-000088, 5-Amino-1MQ demonstrates superior cell permeability and selectivity. Unlike broad-spectrum metabolic modulators such as metformin, it targets a single enzyme (NNMT) with high specificity, making it a valuable tool compound for studying the NNMT-NAD+ axis in metabolic research.

What is the recommended reconstitution protocol for 5-Amino-1MQ?

For this 5mg vial, reconstitute with 1mL of bacteriostatic water to yield a concentration of 5.0mg/mL. Inject the water slowly along the inner wall of the vial — do not shake or agitate. Allow the lyophilized powder to dissolve fully (typically 2-5 minutes). Store reconstituted solution at 2-8°C and use within 30 days.

Is 5-Amino-1MQ legal to buy for research?

5-Amino-1MQ is sold in the United States as a research chemical for laboratory and in-vitro use only. It is not approved by the FDA for human use and is not sold for human consumption. Researchers are responsible for compliance with all applicable federal, state, and institutional regulations.

How is 5-Amino-1MQ reconstituted for research use?

5-Amino-1MQ ships as a lyophilized (freeze-dried) powder and is reconstituted with bacteriostatic or sterile water before use in research. The volume of solvent added determines the working concentration — use the Elyte reconstitution calculator to derive the exact concentration for a given vial size and solvent volume.

Does 5-Amino-1MQ come with a Certificate of Analysis?

Yes. Every batch of 5-Amino-1MQ from Elyte Peptides ships with a third-party Certificate of Analysis (COA) documenting identity and HPLC purity (≥98%), so research results can be traced to a verified lot.

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